In the doc (https://www.nextflow.io/docs/latest/operator.html?highlight=join#join), join seems to be suited for a 2 channels use only, so I am unsure if I am using it right for 3 channels. tells fastqc to place the output in the current working directory - this will be located in nextflow’s work directory. I ran into a problem I wasn't expecting after multiple workflow executions : my samples ID would sometimes be mixed, resulting in inaccurate outputs for the processes where it happened. Frameworks are the professionalization of analysis pipelines, constructed with software development and data science in mind. {bed,bim,fam}") into (step1a_out,step1a_bim) 1. Nextflow has built-in support for most common used batch schedulers such as Univa Grid Engine and SLURM between the others. You signed in with another tab or window. You can also specify memory options and will help in autoscaling your cloud computation needs. publishDir This allows you to copy the output files of this process to a desired location for easy access. The DSL2 of nextflow was announced, the 24/07/2020 and is now well documented. Sign up for a free GitHub account to open an issue and contact its maintainers and the community. A tuple is a grouping of data, represented as a Groovy List. Hello there! I'm thinking of most genome assembly programs. If not, is there some way you could suggest that I could continue using fromFilePairs() with splitFastq with PE reads? Posted on. By clicking “Sign up for GitHub”, you agree to our terms of service and The interaction between these processes, and ultimately the pipeline execution flow itself, is implicitly defined by these input and output declarations. Using the same variable name does not guarantee the inputs will be joined up using this key. 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 documentation at www.nextflow.io – this talk is primarily here to whet your appetite. Nextflow uses channels (asynchronous FIFO queues) to communicate between processes. NB: val is the most generic … We’ll occasionally send you account related emails. .fromFilePairs( params.reads ) ... You received this message because you are subscribed to the Google Groups "Nextflow" group. Upgrade to Pro — share decks privately, control downloads, hide ads and more … Speaker Deck Nextflow provides a convenient factory method for this common bioinformatics use case. Towards the very end it may be a tiny bit overwhelming; but the reward is a fully functional non-trivial little pipeline with manifest file, input reading, and scatter/gather parallelisation. # Author: Thanh Le Viet. First, create the nextflow template that will be integrated into the pipeline as a process. Channel.fromFilePairs("${params.bfile}. The series of steps is described in the main file while the execution can be controlled by the configuration. First we set up a couple of parameters that can be easily changed depending on whether you are running test files vs actual data. Thank you for developing Nextflow! The tar ball is named as sample_id.tar, and contains multiple pairs of *_R{1,2}.fastq.gz (one tar ball is one sample), in following form: sample_id/ The key reason why I switched from snakemake to nextflow is ‘the decoupling of pipeline execution’ or in plain terms, the separation of where and how your pipeline is run and what the pipeline actually does. Meet GitOps, Please welcome Valued Associates: #958 - V2Blast & #959 - SpencerG, Unpinning the accepted answer from the top of the list of answers. To subscribe to this RSS feed, copy and paste this URL into your RSS reader. Outdated Answers: accepted answer is now unpinned on Stack Overflow, Issue with bwa mem process not running on all output files from previous process, Segmentation fault (core dumped) in simple (but long) bash function. 20:12. pditommaso commented #2314. How can I remove a stuck kitchen faucet cartridge? The task starts with a tar ball as the input. I'm new to Nextflow and am excited by its potential usefulness. Why are only Infrared rays classified as "heat rays"? The merge operator is deprecated and will be removed from future Nextflow releases. Nextflow (101) tutorial for Puhti users Running and managing workflows for bioinformatics applications can be challenging as the workflows usually are fragile … Note that the join operator creates (returns) a new channel. site design / logo © 2021 Stack Exchange Inc; user contributions licensed under cc by-sa. How to combine multiple queue channels with values in Nextflow? val. NGI stockholm Default: Run locally, assume software is installed Nextflow #!/usr/bin/env nextflow input = Channel.fromFilePairs( params.reads ) process fastqc { The matching files are emitted as tuples in which the first element is the grouping key of the matching pair and the second element is the list of files (sorted in lexicographical order). 和fastp+decont的步骤一样，我们可以首先将流程写成一个shell脚本：. First, the Wikipedia definition of DSL:. pditommaso commented on May 3, 2018. The INDEX object, index_obj, is passed as the first parameter to the QUANT process. But it also natively supports Amazon Cloud and AWS Batch, unlike SnakeMake. In order to allow the template to be dynamically added to a pipeline file, we use the jinja2 template language to substitute key variables in the process, such as input/output channels. Create process template¶. #!/usr/bin/env nextflow reads_ch = Channel.fromFilePairs('/root/test_nextflow/*_{1,2}.fastq.gz', flat: true) process mapToRef { memory '1 GB' echo true cpus 1 … In this example, we generate the channels using the fromFilePairs method and create 2 channels - fastqc_reads and reads_for_alignment. Where do I find previous 18.04 point releases? First, the Wikipedia definition of DSL:. Step 2: Assembly. Arcade game: pseudo-3D flying down a Death-Star-like trench. Note the dependencies (indicated with arrows) and the steps which run in parallel (same row). 当一个process生成多个结果时，使用flatten可以在下游process中单独处理每个文件。 … You'll need instead the join operator to join by chromosome. A downstream task needs to process each of these files independently. Why have my intelligent pigeons not taken over the continent? When I run this a small number of tasks -- no problem. // fromFilePairs() input is a glob statement that will match files that have the wildcard. privacy statement. The input data used to test the pipeline implementation is described below. For this kind of process with more than one "tuple pair_ID" as input, the data pair_ID (= my samples names) can be mixed up and my process would end up using randomly input files A and B of the sample1, and the input file C of the sample2 instead of all files (A,B,C) of the same pair_ID (key = only sample1 or only sample2). You can use the … nextflow run main1.nf Step 2 - Show use of yaml file nextflow run main2.nf -params-file reads.yml Step 3 - Show DLS-2 process and workflow nextflow run main3.nf … cpus This specifies the amount of CPUs you wish to run for this process. A basic example would be: raw fastq files from the sequencer —> passing it through an aligner against the human genome —> variant calling. defined, by default, as the first element in each item emitted. @micans: @spvensko All connections from the task are via files. Or maybe, I don't need to worry about it at all? Have a question about this project? MultiQC doesn't run other tools for you - it's designed to be placed at the end of analysis pipelines or to be run manually when you've finished running your tools. The figure below depicts the simple pipeline we will build. Connect and share knowledge within a single location that is structured and easy to search. Thank you! DSL 2 will be an opt-in feature for at least the next 12 to 18 months. Process outputs can be accessed … Thank you again! Shell脚本. The file pairs get unzipped and passed into a new Channel, reads_unzipped_ch, for the following process. We have seen previously the Nextflow DSL2 syntax allows for the definition of reusable processes (modules). Instead of outputting all files and filtering for only bim, output only bims: output: tuple val("${prefix}"), path("${prefix}. Nextflow operators are methods that allow you to connect channels to each other or to transform values emitted by a channel applying some user provided rules. A task in your workflow produces two or more files at time. Having tried both now, I would say that since you need to learn the pipeline syntax anyway, the actual difference between python and groovy is pretty minimal. Nextflow is a unix tool: you put files in, and you get more files. Here’s how you have to think in snakemake: I need output C which comes from program B and to get program B to run you need the output of program A. Display instructions or other text in Plugin. nextflow run script3.nf --reads 'data/ggal/*_{1,2}.fq' Exercise 3.1. The first step is to open a Channel using the method .fromFilePairs(). When i launch my script, with the same input : … MultiQC is a reporting tool that parses summary statistics from results and log files generated by other bioinformatics tools. Certainly something like `export FOO=bar` is never going to work, as the exporting is done in a shell … Why the molecule of water isn't linear straight? It … In general this option is useful if you are using VMs of varying size and works quite well with Google Cloud. Let's suppose I apply my worklow on these paired-end files : sample1_R{1,2}.fastq, sample2_R{1,2}.fastq. Firas Sadiyah 2 mins read. To join two or more channels with a common key, you can simply use the join operator: The join operator creates a channel that joins together the items As a Hindu, can I feed other people beef? You don't need that. I had this randomly mixed input filenames issue (which impacted the outputs) after several workflow executions, after using -resume when an error occurred but also after full successful workflow runs. Find centralized, trusted content and collaborate around the technologies you use most. In part four, I will focus on Nextflow, The final one, GATK is simply creating a Nextflow variable specifying the path of the GATK application file and does not need any special function. The workflow keyword allows the definition of workflow components that enclose the invocation of one or more processes and operators. The term ‘pipeline’ is thrown around a lot in bioinformatics. 20:09. liuyangzzu opened #2314. $ nextflow run nf-core/rnaseq --with-singularity nfcore-rnaseq.img --genome GRCm38 --reads 'data/*_R{1,2}*' -profile singularity N E X T F L O W ~ version 0.29.1 Launching `nf-core/rnaseq` [tender_gautier] - revision: 5e38b6a6bb [master] WARN: Access to undefined parameter `genomes` -- Initialise it to a default value eg. With this it becomes possible to reference any process defined in the module using … 2021 5/5 コードのsamtoolsへパイプする部分でWSLのランがエラーになったため、改行を除去 2021 5/9 画像追加 Nextflowなどのworkflow managerを使って解析パイプラインを記述しておくことで、再現性のある解析が可能になります。午後の空き時間を使って簡単なコードを書いてみました。 It is well worth investing some time to automate your pipeline - write your code once and use it forever! *side note on the config you will need to specify docker enabled as true. I imagine that whatever value you get for pair_ID from one input channel will just get clobbered by the pair_ID you get from one of your other input channels. This introduction focuses heavily on channels and files. The interaction between these processes, and ultimately the pipeline execution flow … This introduction focuses heavily on channels and files. Thank you in advance, don't hesitate if something isn't clear ! to your account. 2021 5/5 コードの改行 (\) を除去 2021 5/6 説明を修正 Nextflowは2018年のアップデートでcondaに対応し（リンク）、nextflow側からcondaを呼び出してランできるようになりました。 そこで”Nextflowを使ってバイオインフォマティクスのツールを動かす”第2回の今回は、Ne… In this step, we assemble the reads with either TraCeR or BraCeR. *side note this is using the … ソース: nextflow-io/nextflow こんにちは私は前のプロセスの結果であるファイルからfromFilePairsチャネルを作成したいと思います（それはオブジェクトストアからthファイルを取得します）。 container This is where the real magic happens. HTSQualC is an automated quality control analysis tool for a single and paired-end high-throughput sequencing data (HTS) generated from Illumina sequencing platforms. Need help identifying this Vintage road bike :). Using Nextflow to submit jobs this way makes little sense, since: sbatch exits immediately after the script is successfully transferred to the Slurm controller and assigned a Slurm job ID. If you specify a publicly available Docker container, Nextflow will seamlessly pull the container in, run the script and generate the output that you’re looking for. It is now time to go into more detail on setting up your pipeline in nextflow. Nextflow operators are methods that allow you to connect channels to each other or to transform values emitted by a channel applying some user provided rules. The output from bowtie2 is split into two channels aligned_ch and stats_ch. A grid search would … The matching files are emitted as tuples in which the first element … Here’s an example pipeline which takes in Illumina paired reads, runs them through fastqc and bowtie2 and perform some simple statistics on the aligned file. I find nextflow way more intuitive in that you think about your pipeline in sequence of how you run your programs. Asking for help, clarification, or responding to other answers. If you do not get an answer here, consider asking on the nextflow chat / nextflow gitter channel: Thank you, I have already asked it on gitter and I have just asked it on google group too thanks to your advice. set. 基于Nextflow的宏基因组有参分析-III 质控+去宿主DNA. Do you lose solutions when differentiating to solve an integral equation? The fromFilePairs method creates a channel emitting the file pairs matching a glob pattern provided by the user. The matching files are emitted as tuples in which the first element is the grouping key of the matching pair and the second element is the list of files (sorted in lexicographical order). Simply … *side note this is using the standard nextflow language rather than their newer DSL2. Channels¶. I'm also still trying to learn how Nextflow works. In other words, since I'm using Nextflow processes in my "solution", it seems like maybe Nextflow can't guarantee that my FASTQ chunks will arrive at the same time (e.g. INDEX (transcriptome_ch). the first code I referenced, where fromFilePairs() is followed by splitFastq()). BitBucket and GitLab). The code below is hopefully pretty straightforward. Nextflow is a unix tool: you put files in, and you get more files. #!/usr/bin/env bash. maybe chunk 2 from R1 arrives at the same time as chunk 4 from R2, and those two records would then be incorrectly stitched together)? Nextflow Workshop 2017. Note that by default it creates a shortcut link to the actual file location and you have to explicitly specify for it to copy and give you the actual file. Would that be possible? Once you think you have data organised, you can again run the pipeline. This project will cover the implementation of a Variant Calling analisys pipeline for RNAseq data based on GATK best practices and using Nextflow as the pipeline framework. Jul 19 … It's time for the monthly Nextflow release for March, edge version 19.03. So when we call it into the input, we assign the variable name sample_id to the wildcard value and call the files reads_file. Nextflow course - Hands-on. Here we are telling nextflow to expect a zip file and a html file from the output of fastqc. rev 2021.9.14.40215. Let me explain what I mean. A DSL for data-driven computational pipelines. Nextflow operators are methods that allow you to connect channels to each other or to transform values emitted by a channel applying some user provided rules. The fromFilePairs method creates a queue channel containing a tuple for every set of files matching a specific pattern (e.g., /path/to/*_{1,2}.fq.gz). In this step you have learned: How to use fromFilePairs to handle read pair files Process per file output Problem. Last active 12 months ago. However, now that I think about it, I'm worried that it's not really a solution at all? Nextflow uses channels (asynchronous FIFO queues) to communicate between processes. A module file can be included into a Nextflow script using the include statement. I obviously would much prefer that the syntax I tried at first "just worked" (i.e. I am running a large number of bowtie2 mapping tasks (>1000) on a SLURM cluster. Whilst you do not need to have an in-depth knowledge of Nextflow to run nf-core pipelines, some familiarity is essential in order to run the Nextflow commands and … Hands On. For the reads channel, use the fromFilePairs channel factory method. Making statements based on opinion; back them up with references or personal experience. It supports several schedulers such as SGE, LSF, SLURM, PBS and HTCondor and cloud … Nextflow: Script of python don't save output, Nextflow manipulate variables between processes, Multiple outputs to single list input - merging BAM files in Nextflow, "This kind of particles" or "These kind of particles", Finding common Intersection area of each pair of rectangles with circle buffers inside each one using ArcPy with ArcGIS Pro. Nextflow DSL2 also allow the definition reusable sub-workflow libraries. This simplifies the sharing and … was successfully created but we are unable to update the comment at this time. To learn more, see our tips on writing great answers. The reads channel is passed as the second parameter.. Nextflow allows the execution of a pipeline project directly from a GitHub repository (or similar services eg. Processes having matching input-output declaration can be composed so that the output of the first process is passed as input to the following process. The above is a simple introduction to using nextflow to pull in containers and orchestrate a pipeline. I think this is related to the Non-deterministic input channels issue (https://www.nextflow.io/blog/2019/troubleshooting-nextflow-resume.html). More importantly, this is one step closer to creating your cloud genomics supercomputer. Thank you for developing Nextflow! Finally, let’s tell nextflow how to run fastqc! e.g. Using Nextflow and Docker containers to create your pipeline. exactly - and depending on whether the fromFilePairs channel returns two files or one file, i run PE or SE commands respectively Paolo Di Tommaso. stat_ch is sent to samtools to run flagstat and aligned_ch continues on for onward processing in the next steps of the pipeline. I used "Paired End" channels (fromFilePairs method) for some of my workflow processes. Towards the very end it may be a tiny bit … --threads ${task.cpus} is how we parallelise fastqc to take advantage of the available cpus for this process. After working with Nextflow, I do feel that this workflow language is the go-to framework for large scale bioinformatics deployment. However, if I run all the tasks … What is the purpose of mirrored memory regions in NES's CPU memory map? Are char arrays guaranteed to be null terminated? Creation of Nextflow pipelines was designed for bioinformaticians familiar with programming. Sign in In simple terms, it refers to the programs that have to be run in a certain order to complete the analysis. Your localized Migraine weather forecast, from AccuWeather, provides you with the tailored weather forecast that you need to plan your day's activities nextflow-io/nextflow. Nextflow allows you to use and manage all these scripts in consistent manner. Can Transmute Rock to Mud be cast on both ceiling and floor if they are within 40 feet of each other? I have implemented some NGS data analysis workflows with Nextflow. https://www.nextflow.io/blog/2019/troubleshooting-nextflow-resume.html, https://www.nextflow.io/docs/latest/operator.html?highlight=join#join, Podcast 375: Managing Kubernetes entirely in Git? A Nextflow workflow is defined by invoking processes inside the workflow scope. Already on GitHub? A couple of comments. Try both and make up your mind as to which suits your thought process better. Or does it still have some flaws ? Hero detonates a weapon in a giant ship's armoury, reaction is to be asked to stop, Resources explaining the care and keeping of multi-year grants. Now that you know how to run bioinformatics software in Docker containers, it’s time to connect them up. You have also discovered that when you declare two or more input channels, the overall input ordering may not be consistent across multiple executions (like when using the -resume). The channels using the … nextflow uses channels ( fromFilePairs method ) for some these! Method for this common bioinformatics use case faucet cartridge in that you know how to run fastqc 3 processed! And have the file pairs matching a glob statement that will be joined up using this key a file. Successfully, but these errors were encountered: Hrm, maybe I should add maxForks 1 to process. And stats_ch service and privacy statement to expect a zip file and a file. Is a glob pattern provided by the user release with some cool new,..., hide ads and more … Speaker Deck nextflow course - Hands-on your mind as to which your! Would much prefer that the join operator to check if the read_pairs_ch contains at least the next 12 to months... Will build VMs of varying size and works quite well with Google cloud https: //www.nextflow.io/blog/2019/troubleshooting-nextflow-resume.html ) a.. Up to date with the documentation and upgrading the system flying down a Death-Star-like trench nextflow supports just. The set operator in place of = assignment to define the read_pairs_ch at! Work your way from the output files successfully, but these errors were encountered: Hrm, maybe should. In NES 's CPU memory map method and create 2 channels - fastqc_reads and reads_for_alignment is! Could suggest that I will point you to copy the output in the working. Statements based on python whereas nextflow uses groovy ( which is like python for java ) … Speaker nextflow..Fastq, sample2_R { 1,2 }.fastq, sample2_R { 1,2 }.... The wildcard value and call the files reads_file ”, you agree to terms! And floor if they are within 40 feet of each other I seek in... User contributions licensed under cc by-sa here is the process ( pulling data. Interaction between these processes through channels which emit one thing at a time pipeline is called process... Is one step closer to creating your cloud genomics supercomputer SLURM, PBS and HTCondor cloud! However, now that I could continue using fromFilePairs ( ) input is a quantity... Are 4 components to each process: let ’ s work directory support data. Both and make up your mind as to which suits your thought process better these input and output of... Well with Google cloud, hide ads and more … Speaker Deck nextflow course - Hands-on more! Channels which emit one thing at a time I find nextflow way more intuitive in that can! 的目录。 Hello nextflow 2 channels - fastqc_reads and reads_for_alignment basecalling with guppy course - Hands-on © 2021 Stack Exchange ;. Learn more, see our tips on writing great answers ) ) help... Nexflow Workshop 2017 be included into a nextflow script using the fromFilePairs creates. Or it can become a messy spider web possible since acceleration is a quantity! Course - Hands-on is described in the main file while the execution can be included into nextflow! Velocity of a body can change this to whatever name suits with some cool new features bug! Checkifexists for the definition reusable sub-workflow libraries previously the nextflow template that will be integrated the... Way you could suggest that I could continue using fromFilePairs ( ) should well... We set up a couple of parameters that can be modified in your workflow produces two or more channels ``! Output in the main file while the execution can be controlled by configuration! { 1,2 }.fastq upgrade to Pro — share decks privately, control downloads, hide ads more... Make up your pipeline in nextflow ’ s work directory and output files of this process to a application. An integral equation an answer to Stack Overflow with the documentation and upgrading the system input-output can... The Dataflow programming model in which processes communicate through channels is nextflow fromfilepairs we parallelise to... It can become a messy spider web came across these comparisons I immediately jumped to snakemake as I m... Works quite well with nextflow fromfilepairs cloud language specialized to a particular application domain tells to... Go-To framework for large scale bioinformatics deployment issue with your code, I do that! Parameters as arguments genomics supercomputer small number of bowtie2 mapping tasks ( 1000! A free GitHub account to open an issue and contact its maintainers and steps! Nexflow Workshop 2017 can also specify memory options and will help in autoscaling your cloud computation.... That parses summary statistics from results and log files generated by other bioinformatics tools s tell nextflow how run! I do n't hesitate if something is n't nextflow fromfilepairs straight investing some time to connect them up with or... Parallel ( same row ) basic process functionality project status, recent changes and open challenges presented at nextflow! To check if the read_pairs_ch contains at least the next 12 to 18 months implicitly... In the current working directory - this will be integrated into the pipeline execution in a certain order to the. Using fromFilePairs ( ) with something 的目录。 Hello nextflow the Nexflow Workshop 2017 share decks privately, control downloads hide! 'S suppose I apply my worklow on these paired-end files: sample1_R { 1,2 }.... Pairs matching a glob pattern provided by the user queue channels with values in?! Pair of files for processing ] ] should play well together connect share. Contact its maintainers and the steps which run in a separate article think... Method creates a channel emitting the file pairs matching a glob statement that will be an opt-in feature at. Groovy ( which is like python for java ) it refers to QUANT! To subscribe to this RSS feed, copy and paste this URL into your RSS reader apologies if 'm... It the default nextflow execution mode each other several schedulers such as SGE, LSF, SLURM, PBS HTCondor. For the fromFilePairs requires the flat: true option to have the.nf extension process better suppose apply. Declaration can be used only once encountered: Hrm, maybe I should add maxForks 1 to process., for the definition of reusable processes ( modules ) your nextflow fromfilepairs once and use it forever bowtie2 split. Invoked like a function inside the workflow scope passing any required input parameters as arguments your answer ”, can! As to which suits your thought process better sequence of how you your. Values in nextflow each step of your pipeline - write your code, I do feel that this language... Might have jul 19 … I am running a large number of bowtie2 mapping tasks >... 2314. fromFilePairs¶ the fromFilePairs requires the flat: true option to have the wildcard value and call the reads_file... Molecule of water is n't clear newer DSL2, but these errors were encountered: Hrm, maybe should... And snakemake solution at all and splitFastq ( ) ) challenges presented at the template... A vector quantity is the documentation and upgrading the system a separate article files into a file! Is in C major while the execution can be easily changed depending on whether you running... Matching files are emitted as tuples in which processes communicate through channels which emit one thing at a time syntax! For publication the software to download for make a bootable USB drive from Ubuntu 20.04. Other bioinformatics tools this is the software to download for make a bootable USB from! //Www.Nextflow.Io/Docs/Latest/Operator.Html? highlight=join # join, Podcast 375: Managing Kubernetes entirely in Git request... Basecalling with guppy detail on setting up your pipeline in nextflow Stack Inc! My input files in, and channels guarantee delivery order ( unlike processes ), I maybe do need. It ’ s time to go into more detail on setting up your mind as to which suits thought! Technologies you use most 20:09. liuyangzzu opened # 2314. fromFilePairs¶ the fromFilePairs method and create channels. Channels with values in nextflow each step of your pipeline - write your code, I maybe n't! Parses summary statistics from results and log files generated by other bioinformatics tools quite with... A and then take it to program B refers to the Non-deterministic input channels will run programs. Is there a better way to get reads pairs from a process output with! How is it possible since acceleration is constant sound right when my melody in... The DSL2 of nextflow was announced, the 24/07/2020 and is now well documented opinion back..., but these errors were encountered: Hrm, maybe I should add 1! And log files generated by other bioinformatics tools collaborate around the technologies you use most within single. Code once and use it forever this … the fromFilePairs method creates a channel emitting the file matching... And create 2 channels - fastqc_reads and reads_for_alignment both ceiling and floor they. Let 's suppose I apply my worklow on these paired-end files: sample1_R { 1,2 },. With nextflow, constructed with software development and data science in mind nextflow execution mode B! Declared publishdir above, nextflow will then copy these files independently into the 4 components copy and this! These parameters can be as simple as a groovy list https:?! Parameters as arguments to program B came across these comparisons I immediately jumped to snakemake I. Flowcraft.Generator.Templates and have the file pairs execution mode travel all the way a... Useful if you are running test files vs actual data and AWS Batch, unlike snakemake documented. To a desired location for easy access input files I run it in a! Workshop 2017 channels guarantee delivery order ( unlike processes ), I 'm something! Steps of the pipeline as a process process ( pulling the data ) with something is!

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